Highly Sensitive Detection of Carbaryl Pesticides Using Potentiometric Biosensor with Nanocomposite Ag/r-Graphene Oxide/Chitosan Immobilized Acetylcholinesterase Enzyme

Novel, sensitive, selective, efficient and portable electrochemical biosensors are needed to detect residual contaminants of the pesticide 1-naphthyl methylcarbamate (carbaryl) in environment, food, essential biological fluids. In this work, a study nanocomposite-based Ag reduced graphene oxide (rGO) chitosan (CS) that optimise surface conditions for immobilisation acetylcholinesterase (AChE) enzyme improve performance catalytic is examined. The Ag/rGO/CS nanocomposite membrane was used determine carbaryl using potentiometer transducer. AChE enzyme-based biosensor exhibits good affinity acetylthiocholine chloride (ATCl). It can catalyse hydrolysis ATCl with potential value 197.06 mV, which then oxidised produce detectable rapid response. Under optimal conditions, detected at concentrations linear range 1.0 × 10−8 μg mL−1 limit detection (LoD) 10−9 mL−1. developed wide working concentration range, low concentrations, high sensitivity, acceptable stability, reproducibility simple fabrication, thus providing promising tool residue analysis.